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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetylglucosamine Medium
(N-Acetylglucosamine Medium)
Composition per liter:
N-Acetylglucosamine .................................................................20.0g
Beef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
Triammonium citrate ....................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O..............................................................................0.05g
Tween™ 80................................................................................1.0mL
pH 6.2 ± 0.4 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.2. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of bacteria that can utilize N-acetylglucosamine.