- 定做培養(yǎng)基/定制培養(yǎng)基
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DSMZ培養(yǎng)基383組分A基礎(chǔ)/DSMZ383組分A基礎(chǔ)
Desulfobacterium Medium Base,DSMZ medium 383 Solution A Base,DSMZ Medium 383/DSMZ Medium383/DSMZ 383/DSMZ383
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配套試劑
- 1. A1031 - 丙酮酸鈉/焦葡萄酸鈉 Sodium pyruvate
- 2. SD029 - 微量元素溶液SL-10/微量元素溶液SL10 Trace elements solution SL-10/Trace elements solution SL10
- 3. SD052 - 亞硒酸鹽-鎢酸鹽溶液 Selenite-tungstate solution
- 4. SD104 - 10%硫化鈉溶液（0.5g/支） 10% Na2S solution
- 5. SD092 - Wolin氏維生素溶液(10x) Wolin's vitamin solution(10x)
產(chǎn)品詳情
培養(yǎng)基配方（每升）
Na2SO4 3.0g
KH2PO4 0.2g
NH4Cl 0.3g
NaCl 21.0g
MgCl2·6H2O 3.0g
KCl 0.5g
CaCl2·2H2O 0.15g
Sodium resazurin(刃天青鈉)0.0005g
KH2PO4 0.2g
NH4Cl 0.3g
NaCl 21.0g
MgCl2·6H2O 3.0g
KCl 0.5g
CaCl2·2H2O 0.15g
Sodium resazurin(刃天青鈉)0.0005g
使用說明
稱取本品28.15g于956ml去離子水中，加入微量元素溶液SL-10(SD029)和亞硒酸鹽-鎢酸鹽溶液(SD052)各1支，攪拌溶解，通入80%H2和20%CO2的混合氣體不低于30分鐘使pH低于6.0，裝入?yún)捬跞萜髦校?21℃滅菌15分鐘，冷卻，備用。
制備DSMZ培養(yǎng)基383時(shí)，另制備并加入溶液B(1g碳酸鈉、20ml水，121℃滅菌15分鐘)、溶液C(2.5g丙酮酸鈉、20ml水，過濾除菌)、溶液D(1支SD092)以及溶液E(取4ml SD104)，混合，使用無菌的酸堿溶液調(diào)節(jié)pH至7.0-7.2，備用
制備DSMZ培養(yǎng)基383時(shí)，另制備并加入溶液B(1g碳酸鈉、20ml水，121℃滅菌15分鐘)、溶液C(2.5g丙酮酸鈉、20ml水，過濾除菌)、溶液D(1支SD092)以及溶液E(取4ml SD104)，混合，使用無菌的酸堿溶液調(diào)節(jié)pH至7.0-7.2，備用
特殊說明
383: DESULFOBACTERIUM MEDIUM
1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at
least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same
gas atmosphere. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas
atmosphere. Solutions C and D are prepared under 100% N2 gas and filter-sterilized.
Solution E is autoclaved under 100% N2 gas. To complete the medium appropriate
amounts of solutions B to E are added to the sterile solution A in the sequence as
indicated. Final pH of the medium should be 7.0 – 7.2.
2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution
freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the
beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark.
For DSM 2056: Replace pyruvate with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate, and 0.15
g/l sodium octanoate.
For DSM 3383: Replace pyruvate with indole as substrate. Dissolve 0.30 g of indole in 90
ml water by heating and shaking in a closed bottle under 100% N2 gas atmosphere,
autoclave, then add 7.00 ml of a sterile anoxic stock solution of NaCl (30% w/v) and 0.70
ml of a sterile anoxic stock solution of MgCl2 x 6 H2O (40% w/v). Store the indole-salt
solution in the dark. Reheat and shake before use. Add to sterile medium 30.00 ml/l of the
indole-salt solution in the beginning, and 2 x 30.00 ml/l during growth.
For DSM 3384: The vitamin solution of medium 120 is replaced with 1.00 ml/l of seven
vitamins solution of medium 503. Replace pyruvate with 0.40 g/l Na-benzoate and add
10.00 ml/l clarified rumen fluid (see medium 1310) as growth supplement. Initiation of
growth requires addition of freshly prepared sodium dithionite.
For DSM 4661: Replace pyurvate with resorcinol. During growth the culture is fed once
with the same amount of resorcinol.
For DSM 5091, DSM 9788: Replace pyruvate with 2.00 g/l malonic acid.
For DSM 7044, DSM 7120, DSM 7467, DSM 12567, DSM 13228, DSM 23484: Supplement
medium with 1.00 ml/l seven vitamins solution (see medium 503). Replace pyruvate with
0.40 g/l benzoate and 0.10 g/l yeast extract sterilized separately by filtration and add to
the autoclaved medium from anoxic stock solutions.
For DSM 8540: Replace pyruvate with 0.30 g/l p-hydroxybenzoate.
For DSM 9705: Replace pyruvate with 1.00 g/l Na-glycolate sterilized by filtration.
For DSM 12861, DSM 12883: Replace pyruvate with 1.00 g/l valeric acid.
For DSM 12888: Replace pyruvate with 1.10 g/l Na-butyrate and 1.10 g/l caproic acid.
For DSM 13036: Replace pyruvate with 1.00 g/l betaine.
For DSM 14454: Naphthalene dissolved in heptamethylnonane can be used as substrate
instead of pyruvate.
For DSM 15576, DSM 16219: Replace pyruvate with 1.00 g/l Na-caprylate and supplement
medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved
medium from an anoxic stock solution sterilized by filtration.
For DSM 17291: Replace pyruvate with 3.00 g/l Casamino acids.
For DSM 17477: Replace pyruvate with 1.90 g/l sodium glutamate monohydrate and 1.00
g/l yeast extract.
For DSM 21856: Replace pyruvate with 0.90 ml/l 1-butanol.
For DSM 28890: Replace pyruvate with 0.50 g/l yeast extract and 0.68 g/l sodium formate.
For DSM 100305, DSM 105015: Change amount of pyruvate to 2.20 g/l and add 0.10 g/l
yeast extract.
Solution A
Solution B
Solution C
Solution D
Wolin's vitamin solution (10x) 1.00 ml
Solution E
Selenite-tungstate solution (from medium 385)
Trace element solution SL-10 (from medium 320)
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally
make up to 1000.00 ml.
Wolin's vitamin solution (10x) (from medium 120)
|
Solution A Solution B Solution C Solution D Solution E |
952.00 20.00 20.00 1.00 10.00 |
ml ml ml ml ml |
least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same
gas atmosphere. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas
atmosphere. Solutions C and D are prepared under 100% N2 gas and filter-sterilized.
Solution E is autoclaved under 100% N2 gas. To complete the medium appropriate
amounts of solutions B to E are added to the sterile solution A in the sequence as
indicated. Final pH of the medium should be 7.0 – 7.2.
2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution
freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the
beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark.
For DSM 2056: Replace pyruvate with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate, and 0.15
g/l sodium octanoate.
For DSM 3383: Replace pyruvate with indole as substrate. Dissolve 0.30 g of indole in 90
ml water by heating and shaking in a closed bottle under 100% N2 gas atmosphere,
autoclave, then add 7.00 ml of a sterile anoxic stock solution of NaCl (30% w/v) and 0.70
ml of a sterile anoxic stock solution of MgCl2 x 6 H2O (40% w/v). Store the indole-salt
solution in the dark. Reheat and shake before use. Add to sterile medium 30.00 ml/l of the
indole-salt solution in the beginning, and 2 x 30.00 ml/l during growth.
For DSM 3384: The vitamin solution of medium 120 is replaced with 1.00 ml/l of seven
vitamins solution of medium 503. Replace pyruvate with 0.40 g/l Na-benzoate and add
10.00 ml/l clarified rumen fluid (see medium 1310) as growth supplement. Initiation of
growth requires addition of freshly prepared sodium dithionite.
For DSM 4661: Replace pyurvate with resorcinol. During growth the culture is fed once
with the same amount of resorcinol.
For DSM 5091, DSM 9788: Replace pyruvate with 2.00 g/l malonic acid.
For DSM 7044, DSM 7120, DSM 7467, DSM 12567, DSM 13228, DSM 23484: Supplement
medium with 1.00 ml/l seven vitamins solution (see medium 503). Replace pyruvate with
0.40 g/l benzoate and 0.10 g/l yeast extract sterilized separately by filtration and add to
the autoclaved medium from anoxic stock solutions.
For DSM 8540: Replace pyruvate with 0.30 g/l p-hydroxybenzoate.
For DSM 9705: Replace pyruvate with 1.00 g/l Na-glycolate sterilized by filtration.
For DSM 12861, DSM 12883: Replace pyruvate with 1.00 g/l valeric acid.
For DSM 12888: Replace pyruvate with 1.10 g/l Na-butyrate and 1.10 g/l caproic acid.
For DSM 13036: Replace pyruvate with 1.00 g/l betaine.
For DSM 14454: Naphthalene dissolved in heptamethylnonane can be used as substrate
instead of pyruvate.
For DSM 15576, DSM 16219: Replace pyruvate with 1.00 g/l Na-caprylate and supplement
medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved
medium from an anoxic stock solution sterilized by filtration.
For DSM 17291: Replace pyruvate with 3.00 g/l Casamino acids.
For DSM 17477: Replace pyruvate with 1.90 g/l sodium glutamate monohydrate and 1.00
g/l yeast extract.
For DSM 21856: Replace pyruvate with 0.90 ml/l 1-butanol.
For DSM 28890: Replace pyruvate with 0.50 g/l yeast extract and 0.68 g/l sodium formate.
For DSM 100305, DSM 105015: Change amount of pyruvate to 2.20 g/l and add 0.10 g/l
yeast extract.
Solution A
|
Na2SO4 KH2PO4 NH4Cl NaCl MgCl2 x 6 H2O KCl CaCl2 x 2 H2O Trace element solution SL-10 Selenite-tungstate solution Sodium resazurin (0.1% w/v) Distilled water |
3.00 0.20 0.30 21.00 3.00 0.50 0.15 1.00 1.00 0.50 950.00 |
g g g g g g g ml ml ml ml |
|
Na2CO3 Distilled water |
1.00 20.00 |
g ml |
|
Na-pyruvate Distilled water |
2.50 20.00 |
g ml |
Wolin's vitamin solution (10x) 1.00 ml
Solution E
|
Na2S x 9 H2O Distilled water |
0.40 10.00 |
g ml |
|
NaOH Na2SeO3 x 5 H2O Na2WO4 x 2 H2O Distilled water |
0.50 3.00 4.00 1000.00 |
g mg mg ml |
|
HCl (25%) FeCl2 x 4 H2O ZnCl2 MnCl2 x 4 H2O H3BO3 CoCl2 x 6 H2O CuCl2 x 2 H2O NiCl2 x 6 H2O Na2MoO4 x 2 H2O Distilled water |
10.00 1.50 70.00 100.00 6.00 190.00 2.00 24.00 36.00 990.00 |
ml g mg mg mg mg mg mg mg ml |
make up to 1000.00 ml.
Wolin's vitamin solution (10x) (from medium 120)
|
Biotin Folic acid Pyridoxine hydrochloride Thiamine HCl Riboflavin Nicotinic acid Calcium D-(+)-pantothenate Vitamin B12 p-Aminobenzoic acid (DL)-alpha-Lipoic acid Distilled water |
20.00 20.00 100.00 50.00 50.00 50.00 50.00 1.00 50.00 50.00 1000.00 |
mg mg mg mg mg mg mg mg mg mg ml |
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