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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetomicrobium flavidum Broth
(LMG Medium 71)
Composition per liter:
Pancreatic digest of casein..........................................................10.0g
Lab-Lemco beef extract................................................................3.0g
Yeast extract..................................................................................3.0g
Glucose.........................................................................................2.0g
L -Cysteine hydrochloride..............................................................0.5g
Salt solution.............................................................................40.0mL
Tween™ 80................................................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Salt solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
CaCl 2 ·2H 2 O ................................................................................0.26g
MgSO 4 ·7H 2 O................................................................................0.2g
Preparation of Salt Solution: Add components to 1.0L of dis-
tilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Acetomicrobium flavidum.