[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2019-9-18] [發(fā)布人:] [閱讀次數:] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetomicrobium flavidum Broth
Composition per liter:
Casitone......................................................................................10.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Glucose.........................................................................................2.0g
L -Cysteine·HCl..............................................................................0.5g
Salt solution .............................................................................40.0mL
Tween™ 80................................................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Salt Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
CaCl 2 ·2H 2 O.................................................................................0.26g
MgSO 4 ·7H 2 O................................................................................0.2g
Preparation of Salt Solution: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Acetomicrobium flavidum.