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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acholeplasma Medium
(ATCC Medium 1039)
Composition per liter:
Papaic digest of soybean meal....................................................10.0g
Agar..............................................................................................3.0g
PPLO broth without Crystal Violet........................................900.0mL
Fresh yeast extract solution....................................................100.0mL
pH 7.8 ± 0.2 at 25°C
PPLO Broth without Crystal Violet:
Composition per 900.0mL:
Beef heart, infusion from..........................................................225.0g
Peptone..........................................................................................9.0g
NaCl..............................................................................................4.5g
Source: PPLO broth without Crystal Violet is available as a premixed
powder from BD Diagnostic Systems.
Preparation of PPLO Broth without Crystal Violet:  Add
components to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Mix thoroughly.
Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Re-
move supernatant solution. Adjust pH to 6.6–6.8.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into test tubes or flasks. Autoclave for 10 min at
15 psi pressure–121°C.
Use: For the cultivation and maintenance of Acholeplasma species.